|Voice actor:||Rob Paulsen|
Major Glory had already begun his training to become the symbol of America's spirit and the world's protector as a young child under the tutelage of his Uncle Sam.
Major Glory makes a special appearance in the Powerpuff Girls episode Members Only, where he is shown as leader of the "Association of World Super Men," or A.W.S.M. (awesome) for short. He refused to let the girls join in A.W.S.M. despite Blossom beating him in the Test of Heroism in the Field.
Major Glory's chauvinistic attitude was his downfall when a supervillain named Mascumax took advantage of him and the other male superheroes where he defeated them until he was repelled by the Powerpuff Girls. Afterwards, Major Glory asked the Powerpuff Girls if he and his fellow superheroes can join their team.
- He is a parody of Marvel Comics' Captain America and DC's Superman, having Superman's powers with the patriotic persona of Captain America.
- The Majory Glory featured in The Powerpuff Girls is supposidely a different version from the one featurd on Dexter's Laboratory, due to his team affiliations and chauvinistic attitude; In Dexter's Laboratory, Major Glory was the leader of the Justice Friends, not the Association of World Super Men. The Justice Friends featured at least one female member, Miss Spell, and allowed the membership of the mentally defficient Infraggable Krunk.
- Major Glory's costume in the show is slightly different from his costume from Dexter's Laboratory. In Dexter's Laboratory, he wears a red belt with his pectorals covered by a blue field with 5 white stars, while his abdominals are covered in white. In The Powerpuff Girls, he wears red overshorts with a white belt and gold buckle, and the blue field now covers his abominals as well. The 5 star field on his chest has been reduced to one star. The hem of his gloves also feature small triangular notches not present in his original design.
- Major Glory and Valhallen are the only two Dexter's Laboratory characters ever to have a major role in one episode of The Powerpuff Girls, although Valhallen's screentime was minor at best. The other Dexter's Laboratory characters have not appeared in any of the Powerpuff Girls episodes at all (outside of Dexter's cameos in some episodes of the series).
- Major Glory also has a great dislike of dentists.